Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays
Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays
Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin co...
| Título traducido: | Detección inmunoenzimática de la toxina de la bacteria Clostridium tetani: una alternativa a las pruebas biológicas con ratones |
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| Título de la revista: | Revista de Biología Tropical |
| Primer autor: | Fernando Chaves |
| Otros autores: | Guillermo León; Francisco Hernández Chavarría |
| Palabras clave traducidas: | |
| Idioma: | No especificado |
| Enlace del documento: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/14108 |
| Tipo de recurso: | Documento de revista |
| Fuente: | Revista de Biología Tropical; Vol 54, No 2 (Año 2006). |
| DOI: | http://dx.doi.org/10.15517/rbt.v54i2.14108 |
| Entidad editora: | Universidad de Costa Rica |
| Derechos de uso: | Reconocimiento (by) |
| Materias: | Ciencias de la Vida --> Biología |
| Resumen: | Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice. |
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| Resumen traducido: | Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice. |
