Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi

Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi
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Amazonian açai and food dyes for staining arbuscular- micorrhizal fungi

Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with...

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Translated title: Açaí da Amazônia como corante de fungos micorrízicos arbusculares
Journal Title: Pesquisa Florestal Brasileira
Main Author: Aline Lourdes Martins Silva
Other Authors: Marcos Diones Ferreira Santana;
John César de Jesus Pereira;
Milena Pupo Raimam;
Ulisses Brigatto Albino
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Language: English
Get full text: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/798
Resource type: Journal article
Source: Pesquisa Florestal Brasileira; Vol 35, No 84 (Year 2015).
DOI: http://dx.doi.org/10.4336/2015.pfb.35.84.798
Publisher: Embrapa Florestas
Usage rights: Reconocimiento - NoComercial - SinObraDerivada (by-nc-nd)
Subjects: Sciences --> Biodiversity Conservation
Sciences --> Biology
Sciences --> Environmental Sciences
Applied Sciences --> Agriculture, Multidisciplinary
Applied Sciences --> Agronomy
Applied Sciences --> Forestry
Abstract: Arbuscular mycorrhizae microscopy requires differential staining of typical structures. Dyes employed, such as trypan blue, pose risks to health and environment. Alternative dyes such as pen ink and aniline have variable coloring efficiency. In this work, Brachiaria decumbens roots, discolored with caustic soda (NaOH), were stained with açai, annatto, saffron, trypan blue and pen inks. There were significant differences among dyes regarding stained mycorrhizal structures and pictures quality. Acai was considered the best alternative dye, with similar results to trypan blue.
Translated abstract: A microscopia de Micorrizas Arbusculares requer coloração diferencial de estruturas típicas. Alguns corantes empregados, como Trypan Blue, oferecem riscos à saúde e ambiente. Corantes alternativos como tintas de caneta e anilina foram propostos, porém, com eficácia de coloração variável. Neste trabalho o descolorante de raízes KOH foi substituído por Soda Cáustica e foram testados os corantes naturais Açaí, Urucum e Açafrão, comparados a Trypan Blue e tintas de caneta. Houve diferença significativa entre os corantes quanto às estruturas micorrízicas coradas e qualidade de imagens fotografadas. Açaí foi considerado o melhor corante alternativo, com resultados similares ao Trypan Blue.